DNA
HBS

Part:BBa_K4450006:Design

Designed by: Andreas Sagen   Group: iGEM22_UiOslo_Norway   (2022-09-12)


UDP-N-acetylglucosamine synthesis


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1034
    Illegal SpeI site found at 5782
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal SpeI site found at 5782
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 368
    Illegal BglII site found at 1031
    Illegal BglII site found at 4268
    Illegal BamHI site found at 4799
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1034
    Illegal SpeI site found at 5782
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1034
    Illegal SpeI site found at 5782
    Illegal AgeI site found at 173
    Illegal AgeI site found at 913
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 5705
    Illegal SapI site found at 3371
    Illegal SapI.rc site found at 1245


Design Notes

See design page in each individual basic part.

Source

Saccharomyces Cerevisiae ATCC 201388

References